1 resultado para Genetic and epigenetic alterations
em Repositório Institucional da Universidade Federal de São Paulo - UNIFESP
Filtro por publicador
- Aberdeen University (4)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (2)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (24)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (3)
- Aquatic Commons (10)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (7)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (3)
- Aston University Research Archive (4)
- Biblioteca de Teses e Dissertações da USP (1)
- Biblioteca Digital | Sistema Integrado de Documentación | UNCuyo - UNCUYO. UNIVERSIDAD NACIONAL DE CUYO. (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (31)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (9)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (6)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (85)
- Brock University, Canada (2)
- Bucknell University Digital Commons - Pensilvania - USA (1)
- CaltechTHESIS (5)
- Cambridge University Engineering Department Publications Database (2)
- CentAUR: Central Archive University of Reading - UK (33)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (12)
- Cochin University of Science & Technology (CUSAT), India (1)
- Coffee Science - Universidade Federal de Lavras (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (4)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (1)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (42)
- Department of Computer Science E-Repository - King's College London, Strand, London (4)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (2)
- Digital Archives@Colby (1)
- Digital Commons at Florida International University (3)
- DigitalCommons - The University of Maine Research (1)
- DigitalCommons@The Texas Medical Center (29)
- DigitalCommons@University of Nebraska - Lincoln (2)
- DRUM (Digital Repository at the University of Maryland) (1)
- Duke University (10)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (12)
- Glasgow Theses Service (3)
- Greenwich Academic Literature Archive - UK (1)
- Helda - Digital Repository of University of Helsinki (45)
- Hospitais da Universidade de Coimbra (2)
- Indian Institute of Science - Bangalore - Índia (11)
- Instituto Gulbenkian de Ciência (1)
- Instituto Nacional de Saúde de Portugal (2)
- Instituto Politécnico de Viseu (1)
- Instituto Politécnico do Porto, Portugal (2)
- Instituto Superior de Psicologia Aplicada - Lisboa (1)
- National Center for Biotechnology Information - NCBI (44)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (4)
- Portal de Revistas Científicas Complutenses - Espanha (1)
- Publishing Network for Geoscientific & Environmental Data (17)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (66)
- Queensland University of Technology - ePrints Archive (69)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (1)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (3)
- Repositório Institucional da Universidade de Aveiro - Portugal (6)
- Repositório Institucional da Universidade Federal de São Paulo - UNIFESP (1)
- Repositório Institucional dos Hospitais da Universidade Coimbra (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (218)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (1)
- SAPIENTIA - Universidade do Algarve - Portugal (7)
- Savoirs UdeS : plateforme de diffusion de la production intellectuelle de l’Université de Sherbrooke - Canada (1)
- School of Medicine, Washington University, United States (5)
- Universidad de Alicante (1)
- Universidad del Rosario, Colombia (5)
- Universidad Politécnica de Madrid (6)
- Universidade Complutense de Madrid (2)
- Universidade de Lisboa - Repositório Aberto (2)
- Universidade Federal do Pará (5)
- Universidade Federal do Rio Grande do Norte (UFRN) (2)
- Universidade Metodista de São Paulo (2)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (3)
- Université de Lausanne, Switzerland (12)
- Université de Montréal (1)
- Université de Montréal, Canada (10)
- Université Laval Mémoires et thèses électroniques (1)
- University of Michigan (5)
- University of Queensland eSpace - Australia (23)
- WestminsterResearch - UK (3)
Resumo:
The large-scale production of cardiomyocytes is a key step in the development of cell therapy and tissue engineering to treat cardiovascular diseases, particularly those caused by ischemia. the main objective of this study was to establish a procedure for the efficient production of cardiomyocytes by reprogramming mesenchymal stem cells from adipose tissue. First, lentiviral vectors expressing neoR and GFP under the control of promoters expressed specifically during cardiomyogenesis were constructed to monitor cell reprogramming into precardiomyocytes and to select cells for amplification and characterization. Cellular reprogramming was performed using 5'-azacytidine followed by electroporation with plasmid pOKS2a, which expressed Oct4, Sox2, and Klf4. Under these conditions, GFP expression began only after transfection with pOKS2a, and less than 0.015% of cells were GFP(+). These GFP(+) cells were selected for G418 resistance to find molecular markers of cardiomyocytes by RT-PCR and immunocytochemistry. Both genetic and protein markers of cardiomyocytes were present in the selected cells, with some variations among them. Cell doubling time did not change after selection. Together, these results indicate that enrichment with vectors expressing GFP and neoR under cardiomyocyte-specific promoters can produce large numbers of cardiomyocyte precursors (CMPs), which can then be differentiated terminally for cell therapy and tissue engineering.